We have determined that a biological molecule can be physically immobilized on a polymer containing an azobenzene (azopolymer) using irradiating light. We immobilized antibodies and antigens on the surface of an azopolymer coated glass slide (antibody array) to establish “on-chip ELISAs”. The assays used the flat-surface of a glass slide and could be applied to both sandwich and competitive ELISAs. The sensitivity and accuracy of the on-chip ELISA were similar to a conventional ELISA using a polystyrene plate. Using the assay system, we proved that representative oxidative-biomarkers could be simultaneously measured from uL of urine. That should realize low-cost study on animal or human, and accelerate development of health-promoting foods. So, this new concept antibody array has promising applications in proteomic studies, and could be used to examine biomarkers to investigate health-promoting food.
CITATION STYLE
Hoshino, F., Watanabe, O., Wu, X., Takimoto, Y., & Osawa, T. (2014). Low-cost and easy-to-use “on-chip ELISA” for developing health-promoting foods. Sub-Cellular Biochemistry, 77, 151–161. https://doi.org/10.1007/978-94-007-7920-4_13
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