Skip to main content

Analysis of cell wall teichoic acids in Staphylococcus aureus

9Citations
Citations of this article
21Readers
Mendeley users who have this article in their library.
Get full text

Abstract

Most bacterial cells are surrounded by a surface composed mainly of peptidoglycan (PGN), a glycopolymer responsible for ensuring the bacterial shape and a telltale molecule that betrays the presence of bacteria to the host immune system. In Staphylococcus aureus, as in most gram-positive bacteria, peptidoglycan is concealed by covalently linked molecules of wall teichoic acids (WTA)—phosphate rich molecules made of glycerol and ribitol phosphates which may be tailored by different amino acids and sugars. In order to analyze and compare the composition of WTA produced by different S. aureus strains, we describe methods to: (1) quantify the total amount of WTA present at the bacterial cell surface, through the determination of the inorganic phosphate present in phosphodiester linkages of WTA; (2) identify which sugar constituents are present in the assembled WTA molecules, by detecting the monosaccharides, released by acid hydrolysis, through an high-performance anion exchange chromatography analysis coupled with pulsed amperometric detection (HPAEC-PAD) and (3) compare the polymerization degree of WTA found at the cell surface of different S. aureus strains, through their different migration in a polyacrylamide gel electrophoresis (PAGE).

Cite

CITATION STYLE

APA

Covas, G., Vaz, F., Henriques, G., Pinho, M. G., & Filipe, S. R. (2016). Analysis of cell wall teichoic acids in Staphylococcus aureus. In Methods in Molecular Biology (Vol. 1440, pp. 201–213). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3676-2_15

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free