Photoreversible Patterning of Biomolecules within Click‐Based Hydrogels

Abstract

Lighting the way: Biochemical cues have been reversibly patterned into hydrogels with full spatiotemporal control by using two photochemical reactions. The hydrogel is conjugated with a peptide by a thiol-ene photoaddition reaction that is initiated by visible light. Subsequent, selective photocleavage of an o-nitrobenzyl ether with UV light enables dynamic presentation of the peptide to cells with control in three dimensions. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.