GC-MS Analysis of Volatile Plant Secondary Metabolites

Abstract

Knowledge of the identity and relative amounts of the volatile substances released by plants is of great importance to several fields of basic and applied research in biology, chemistry and may other disciplines. Obtaining this knowledge requires overcoming many analytical challenges posed by these complex mixtures, because they normally present large variations in component amounts, chemical structures and functionalities. Gas chromatography (GC) is recognized as the most suitable technique to find out how many components and in what proportion there are in a complex mixture of volatile compounds. When it is coupled to mass spectrometry (GC-MS), additional information arises about each separated compound molecular mass, elemental composition (when high resolution mass spectrometry is used), functional groups, and in certain cases, molecular geometry and spatial isomerism. This chapter covers the most determinant factors which affect biogenic volatile compound (BVOC) GC-MS analysis. Sample preparation, chromatographic separation and mass spectrometry are the main parts explained within the framework of BVOC analysis and practical examples of their application to flower scent and essential oil analysis are presented