Effects of multienzyme complex and probiotic supplementation on the growth performance, digestive enzyme activity and gut microorganisms composition of snakehead (Channa argus)

Abstract

The major objective of this study conducted for 7 weeks was to investigate the influence of supplementation with multienzyme complex and probiotic and their interaction effects on the growth performance, body composition, serum biochemical parameters and gut microorganism composition of snakehead (Channa argus). There were four treatments in this experiment coded as control, probiotic (Bacillus amyloliquefaciens), multienzyme (amylase, acid protease and papain) and pro-multi (B. amyloliquefaciens and amylase, acid protease and papain), respectively. The results demonstrated that snakeheads on the multienzyme resulted in better growth performance in terms of final body weight (FBW), weight gain ratio (WGR) and feed conversion ratio (FCR) significantly (p < 0.05) than those on the control diet, while they did not (p > 0.05) on probiotic and pro-multi. Snakeheads receiving multienzyme increased significantly (p < 0.05) the serum parameters, including albumin, total protein and AKP and probiotic increased AKP and lysozyme meanwhile decreased the LDL-C significantly (p < 0.05) compared with control. Analysis of the composition of gut microorganisms showed that four dominant microorganisms were Spirochaetes, Proteobacteria, Firmicutes and Bacteroidetes. Probiotic could enhance the richness and diversity of gut microorganisms in snakeheads, furthermore revealing a means of evaluating the relationship between feed supplementation and microorganisms.