Pengaruh Pemberian Probiotik Lactobacillus reuteri Terhadap Presentase Sel T regulator dan Sel T helper 22 Pada Limpa Mencit Nifas yang Diinduksi Bakteri Staphylococcus aureus

Abstract

Postpartum infection is an infection that occurs due to bacteria in the genital tract after delivery, due to injury to the cervical area, vulva, vagina, and perineum during labor. Studies explain the bacteria that often cause female genital tract infection is Staphylococcus aureus and there is trauma or injury. Widespread bacterial resistance to existing drugs, encouraging to extract antibacterial sources from other substances, such as use probiotics. Probiotics are described as non-pathogenic microorganisms or bacterial components in food supplementation, including bacterial flora classes, which have health benefits for disease prevention and treatment, when in sufficient quantities. This study aims to prove whether there is an effect of giving Lactobacillus reuteri Treg cells percentage and Th22 cell percentage in the staphylococcus aureus-induced lymph node spleen. The study uses pure post test-only randomized control-group experimental design which utilizes 40 mice (Blb/c) and gestasional aged 13 days which are divided into 8 sample groups. Dosis Lactobacillus reuteri 1x10 10 CFU/mice in 250μl via a oral sonde (a gavaging needle). Dosis Staphyloccus aureus 5x10 7 CFU/mice in 200μl via spuit without needle per vaginal. Treg cells percentage and Th22 cell percentage were analysed for Flow cytometry. This study showed that the probiotic results of Lactobacillus reuteri were not proven to affect Treg cells percentage and Th22 cell percentage on the first day of postpartum. Provision of probiotics Lactobacillus reuteri until the third day postpartum proved to affect percentage Treg cells percentage and Th22 cells percentage.