Analyzing meiotic chromosomes in rice

Abstract

Development of techniques to analyze pachytene chromosomes has greatly overcome most of the dif fi culties in cytological studies of rice chromosomes caused by their small size. Visualization of meiotic chromosomes has now become routine in cytogenetic studies in this species. This chapter provides protocols on basic meiotic chromosome preparation, FISH analysis, and immunocytology in rice. © 2013 Springer Science+Business Media New York.