Stress Sensitivity of Correlation between POD and PPO Activities in Plants

Abstract

In the leaf extracts of two plant species (Quercus robur L. and Phaseolus vulgaris L.), peroxidase (POD) and polyphenol oxidase (PPO) activities have been measured by the methods of Shannon et al (1966) and Flurkey and Jen (1978). The oxidative activities regarded as semi-empirical biochemical variables have distributions in the plant foliage and between them a linear correlation has been observed. In this work the resultant oxidative activities of POD and PPO isoenzymes of plant foliages, the measuring uncertainties of their values and their correlation are interpreted. For the effects of cold shock and lack of illumination, significant alterations in the correlation have been revealed that are also reflected by the parameters of the regression. The correlation of POD and PPO has been established to be stress sensitive by the application of covariance analysis (ANCOVA).