Cytokine Production by the Murine Macrophage Cell Line J774.1 after Exposure to Lactobacilli

Abstract

Eleven strains of lactobacilli were tested for their ability to induce the murine macrophage-like cell line J774.1 to secrete cytokines. Some of the bacteria tested induce the production of interleukin(IL) 6, IL-12, and tumor necrosis factor α (TNF-α) by J774.1 cells. Seven strains also induced the production of IL-10. However, no IL-1β was produced. Lactobacillus acidophilus TMC 0356 significantly induced the production of more IL-6, IL-10, IL-12, and TNF-α than the other bacteria tested (p<0.0001; ANOVA). These results suggest that lactobacilli can activate macrophages to secrete both inflammatory and anti-inflammatory cytokines. Selected strains might be used to bring about pro or antiinflammatory immune reactions. © 2002 by Japan Society for Bioscience, Biotechnology, and Agrochemistry.