Comprehensive identification and profiling of antigens in immune complexes (IC-antigens) is useful to provide insights into pathophysiology and could form the basis for novel diagnostic and treatment strategies for many immune-related diseases. Immune complexome analysis is the method for comprehensively identifying and profiling IC-antigens in biological fluids (such as serum and cerebrospinal fluid). Here, we describe an IC-antigen detection method; specifically, ICs in biological fluids are captured by using protein G- or protein A-coated beads, are subjected to papain-digestion, elution, and tryptic digestion, and are analyzed by nano-liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS).
CITATION STYLE
Aibara, N., & Ohyama, K. (2019). Identification of Antigens in Immune Complexes. In Methods in Molecular Biology (Vol. 2024, pp. 333–338). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9597-4_22
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