Identification of chemical components from essential oils and aqueous extracts of some medicinal plants and their nematicidal effects on Meloidogyne incognita

Abstract

Essential oils from aerial parts of Achillea wilhelmsii, Tanacetum polycephalum and Teucrium polium were isolated by using Clevenger-type apparatus and tested at different concentrations for their nematicidal activity against the second stage juvenile (J2) of Meloidogyne incognita in vitro condition. The chemical components of the essential oils and seed extracts of each plant (0.2 g) were extracted with maceration with methanol/acetic acid mixture (85:15, v/v). Analysis was done by Gas Chromatography, GC-Mass Spectrometry) and HPLC. Identified chemical components were tested after this on J2 of M. incognitain. Nuclear magnetic resonance spectroscopy was done to investigate the properties of organic molecules by drawing their spectrum using Broker AVANCE AQS-300 MHz.