Diagnostic and prognostic value of an immunofluorescent assay for melioidosis

15Citations
Citations of this article
23Readers
Mendeley users who have this article in their library.

Abstract

Melioidosis caused by Burkholderia pseudomallei is endemic in southeast Asia. The clinical manifestations range from wound infections to acute septicemia. In some cases, recurrence can also occur following complete recovery. Case fatality rates are high and a major factor is the delay in the culture and identification of the bacterium. An immunofluorescent assay (IFAT) using whole-cell antigen for the detection of total antibodies to B. pseudomallei was tested with 650 sera. Using a cut-off value of 1:80, 66 sera from culture-confirmed cases were positive with titers ≥320. In another 523 sera from patients in which no other etiology could be found, 149 (23.4%) were positive. To monitor disease activity, persistence of antibody levels was investigated on 61 serial sera samples collected from 14 other confirmed cases on follow-up visits while on oral maintenance therapy. The IFAT demonstrated a reduction in titers in cases of localized infections, suggesting that either the infection was being resolved or arrested while septicemic patients maintained high IFAT titers on follow-up, suggesting the possibility of continuous sequestration of antigen from an intracellular source.

References Powered by Scopus

Melioidosis: A Major Cause of Community-Acquired Septicemia in Northeastern Thailand

481Citations
N/AReaders
Get full text

Relapse in Melioidosis: Incidence and Risk Factors

206Citations
N/AReaders
Get full text

Septicaemic melioidosis: A review of 50 cases from Malaysia

133Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Development and evaluation of polymerase chain reaction assay to detect Burkholderia genus and to differentiate the species in clinical specimens

32Citations
N/AReaders
Get full text

Burkholderia Pseudomallei Causing Bone and Joint Infections: A Clinical Update

24Citations
N/AReaders
Get full text

Detection of spleen abscesses facilitates diagnosis of melioidosis in Malaysian children

10Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Vadivelu, J., & Puthucheary, S. D. (2000). Diagnostic and prognostic value of an immunofluorescent assay for melioidosis. American Journal of Tropical Medicine and Hygiene, 62(2), 297–300. https://doi.org/10.4269/ajtmh.2000.62.297

Readers over time

‘09‘11‘14‘16‘17‘18‘19‘20‘21‘2302468

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 11

73%

Researcher 2

13%

Professor / Associate Prof. 1

7%

Lecturer / Post doc 1

7%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 6

43%

Medicine and Dentistry 6

43%

Decision Sciences 1

7%

Linguistics 1

7%

Save time finding and organizing research with Mendeley

Sign up for free
0