Comparison Of L-Dopa Content In Three Species Of Genus Mucuna By Different Extraction Techniques

Abstract

In the present study, attempts are made to develop suitable method(s) for extraction of L-DOPA from the powdered seeds of 3 species of Mucuna using different solvents and conditions. The seed powder of both plants was subjected to 6 different extraction methods, with different solvent ratios. All the extracts were analyzed using RP-HPLC and was validated according to The International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use guidelines. The L-DOPA extraction was best with Methanol Water mixture in a cold maceration technique and overall gives good extraction efficiency in all the three plants giving concentrations of 5.03%, 13.36 % and 16.78% of L-DOPA in Mucuna gigantea, Mucuna pruriens and Mucuna monosperma, respectively. The present investigation was done to study the extraction efficiency of various extraction methods of L-DOPA content in seed extracts of Mucuna and compare it.