El glufosinato de amonio altera la calidad y el adn de los espermatozoides de ratón

Abstract

Glufosinate ammonium (GLA) is an organophosphorus herbicide used to control weeds. GLA exposure increases premature parturition, abnormalities, and stillbirths in pregnant females, as well as a delay in preimplantation zygote development. However, it is unkown whether GLA targets sperm cells during last stages of development and maturation, and if it damages sperm quality. This study evaluated the effects of GLA exposure on sperm quality and sperm DNA following exposure during two crucial stages of sperm development, the spermatid and final maturation. Adult male ICR mice were administered daily with 5, 7.5 and 10 mg/kg/d GLA for 5-or 15-days of treatment (dt), and euthanized 24 h post-treatment. Spermatozoa were collected from cauda epididymis-vas deference and were then used to evaluate sperm quality and DNA damage. At 5-or 15-dt, the doses of 5, 7.5 and 10 mg/kg/d GLA significantly decreased sperm quality parameters. Also, at 5-dt, the dose of 10 mg/kg/d GLA significantly increased the % DFI (DNA fragmentation index), whereas at 15-dt the doses of 7.5 and 10 mg/kg/d significantly increased the mean of % DFI and %HDS (For its initials in English: High DNA stainability). These data suggest that spermatid and mature sperm cells are targeted by GLA.