Advances in DNA synthesis and assembly technology allow for the high-throughput fabrication of hundreds to thousands of multi-part genetic constructs in a short time. This allows for rapid hypothesis-testing and genetic optimization in multi-gene biological systems. Here, we discuss key considerations to design and implement an algorithmic DNA assembly pipeline that provides the freedom to change nearly any design variable in a multi-gene system. In addition to considerations for pipeline design, we describe protocols for three useful molecular biology techniques in plasmid construction.
CITATION STYLE
Hsu, S. Y., & Smanski, M. J. (2018). Designing and implementing algorithmic DNA assembly pipelines for multi-gene systems. In Methods in Molecular Biology (Vol. 1671, pp. 131–147). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7295-1_9
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