The studies of oncogene dependence are aimed to understand an unfortunate and puzzling aspect of targeted anticancer treatments-their progression to drug resistance. Drug resistance develops from a pool of cells that survive the original treatment, called minimal residual disease. Mouse models based on tetracycline-dependent expression of transgenic oncogenes are used to imitate targeted oncogene blockade and to reproduce minimal residual disease in humans. Here we describe a novel method for generating oncogene-dependent mammary tumors using somatic transfer of transactivator-containing retroviruses into transgenic mice with tetracycline-dependent oncogenes and a method for measuring continuous mitotic activity in epithelial cells in real time.
CITATION STYLE
Botta, C., Darini, C., Darrasse-Jèze, G., & Podsypanina, K. (2015). Methods to study primary tumor cells and residual tumor cells in mouse models of oncogene dependence. Methods in Molecular Biology (Clifton, N.J.), 1267, 381–394. https://doi.org/10.1007/978-1-4939-2297-0_19
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