Aconitate hydratase

Abstract

Twenty enzyme loci were mapped on the three linkage groups of Aedes triseriatus using intraspecific and interspecific matings. Large numbers of single-pair forced matings were made among field-collected A. triseriatus. Parents with appropriate isozyme linkage genotypes were identified and the progeny analyzed using standard electrophoretic procedures. Interspecific data were obtained by performing single­-pair forced matings between A. triseriatus and either A. hendersoni or A. brelandi and then backcrossing to one of the parental species. Interspecific recombination values were adjusted to compensate for reduced chiasmata (and crossovers) in progeny of interspecific crosses. Four loci-Aat2, Me, Idh1, and Mpi-were asso­ciated with sex on linkage group (LG) I. The LG I map was about 24% longer than the predicted length of 62 map units. Eleven loci-Gpi, Hk4,Odh, Est2, Pgm, Sod1, Gpd, Had, Aco2, Idh2, and Est5--were assigned to LG II and spanned approximately 60 map units. Five loci-Mdh2, Pgd, Aat1, Gapd, and Fum-were assigned to LG III, but exact positions and distances of loci were not definitely established. The linkage relationships of enzyme loci of A. triseriatus were compared to maps of five other Aedes species in four subgenera. Map differences indicated several major inversions and translocations that separated the subgenera. In addition, several linkage groups appeared to have been conserved during Aedes subgeneric divergence.