This chapter deals with imaging transparent objects (e.g., live unstained cells) in the light microscope. Most biological specimens are transparent (i.e., in the light microscope the image is of constant intensity across the field of view and there is no contrast). Transparent specimens, such as thin layers of cells, are called phase objects.
CITATION STYLE
Masters, B. R. (2020). Phase Microscopy to Enhance Contrast. In Springer Series in Optical Sciences (Vol. 227, pp. 213–228). Springer. https://doi.org/10.1007/978-3-030-21691-7_12
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