Transposon mutagenesis is one of the most widely used strategies to generate a large number of random mutations within a bacterial genome and then to precisely identify the mutated sites. The generation of sequence-defined transposon mutant libraries that are composed of a collection of different mutants, each containing a single transposon insertion mutation within nearly all of the nonessential genes within the genome, is a rapid and reliable way to enhance the study of gene function. In this chapter, we describe the process to generate a sequence-defined transposon mutant library in Staphylococcus aureus utilizing the mariner-based bursa aurealis transposon.
CITATION STYLE
Endres, J. L., Yajjala, V. K., Fey, P. D., & Bayles, K. W. (2019). Construction of a Sequence-Defined Transposon Mutant Library in Staphylococcus aureus. In Methods in Molecular Biology (Vol. 2016, pp. 29–37). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9570-7_3
Mendeley helps you to discover research relevant for your work.