RNA interference (RNAi) is one of the most commonly used procedures for gene targeting in today's cutting edge technology and has great potential for use in clinical therapy. Using a plasmid construct that exogenously expresses short-hairpin RNAs (shRNAs) targeting a desired gene transcript not only helps to study the downstream effects of a gene product but also offers an alternative to viral vectors for gene therapy. Using a plasmid vector to knockdown a gene allows for long-term and permanent gene knockdown, without the need to generate knockout genotypes. Here, we detail the methodology for constructing a plasmid targeting the human telomerase reverse transcriptase (hTERT) gene through RNAi using the Ambion pSilencer system.
CITATION STYLE
Lai, S. R., Andrews, L. G., & Tollefsbol, T. O. (2007). RNA interference using a plasmid construct expressing short-hairpin RNA. Methods in Molecular Biology (Clifton, N.J.), 405, 31–37. https://doi.org/10.1007/978-1-60327-070-0_4
Mendeley helps you to discover research relevant for your work.