Developing an advanced gut on chip model enabling the study of epithelial cell/fibroblast interactions

55Citations
Citations of this article
129Readers
Mendeley users who have this article in their library.

Abstract

Organoids are widely used as a model system to study gut pathophysiology; however, they fail to fully reproduce the complex, multi-component structure of the intestinal wall. We present here a new gut on chip model that allows the co-culture of primary epithelial and stromal cells. The device has the topography and dimensions of the mouse gut and is based on a 3D collagen I scaffold. The scaffold is coated with a thin layer of laminin to mimic the basement membrane. To maintain the scaffold structure while preserving its cytocompatibility, the collagen scaffold was rigidified by threose-based post-polymerization treatment. This treatment being cytocompatible enabled the incorporation of primary intestinal fibroblasts inside the scaffold, reproducing the gut stromal compartment. We observed that mouse organoids, when deposited into crypts, opened up and epithelialized the scaffold, generating a polarized epithelial monolayer. Proper segregation of dividing and differentiated cells along the crypt-villus axis was achieved under these conditions. Finally, we show that the application of fluid shear stress allows the long-term culture of this intestinal epithelium. Our device represents a new biomimetic tool that captures key features of the gut complexity and could be used to study gut pathophysiology.

References Powered by Scopus

Biochemistry and molecular cell biology of diabetic complications

7630Citations
N/AReaders
Get full text

Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche

5398Citations
N/AReaders
Get full text

A global double-fluorescent cre reporter mouse

2662Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Mechanical compartmentalization of the intestinal organoid enables crypt folding and collective cell migration

114Citations
N/AReaders
Get full text

3D in vitro morphogenesis of human intestinal epithelium in a gut-on-a-chip or a hybrid chip with a cell culture insert

106Citations
N/AReaders
Get full text

Advances in Spheroids and Organoids on a Chip

64Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Verhulsel, M., Simon, A., Bernheim-Dennery, M., Gannavarapu, V. R., Gérémie, L., Ferraro, D., … Descroix, S. (2021). Developing an advanced gut on chip model enabling the study of epithelial cell/fibroblast interactions. Lab on a Chip, 21(2), 365–377. https://doi.org/10.1039/d0lc00672f

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 54

70%

Researcher 18

23%

Professor / Associate Prof. 4

5%

Lecturer / Post doc 1

1%

Readers' Discipline

Tooltip

Engineering 25

41%

Biochemistry, Genetics and Molecular Bi... 24

39%

Agricultural and Biological Sciences 7

11%

Chemistry 5

8%

Save time finding and organizing research with Mendeley

Sign up for free