Cellulases from insects

Abstract

Bioethanol is currently produced by the fermentation of sugary and starchy crops, but waste plant biomass is a more abundant source because sugars can be derived directly from cellulose. One of the limiting steps in the biomass-toethanol process is the degradation of cellulose to fermentable sugars (saccharification). This currently relies on the use of bacterial and/or fungal cellulases, which tend to have low activity under biorefinery conditions and are easily inhibited. Some insect species feed on plant biomass and can efficiently degrade cellulose to produce glucose as an energy source. Although insects were initially thought to require symbiotic relationships with bacteria and fungi to break down cellulose, several species in the orders Dictyoptera, Orthoptera, and Coleoptera have now been shownto produce their own cellulases in the midgut or salivary glands, and putative cellulose genes have been identified in other orders. Insect cellulases often work in concert with cellulases provided by symbiotic microbiota in the gut to achieve efficient cellulolysis.We discuss the current status of insect cellulases and potential strategies that could be used to find novel enzymes and improve their efficiency.