Microbial Biomarkers Identification for Human Gut Disease Prediction using Microbial Interaction Network Embedded Deep Learning

Abstract

Human gut microorganisms are crucial in regulat-ing the immune system. Disruption of the healthy relationship between the gut microbiota and gut epithelial cells leads to the development of diseases. Inflammatory Bowel Disease (IBD) and Colorectal Cancer (CRC) are gut-related disorders with complex pathophysiological mechanisms. With the massive availability of microbiome data, computer-aided microbial biomarker discovery for IBD and CRC is becoming common. However, microbial in-teractions were not considered by many of the existing biomarker identification methods. Hence, in this study, we aim to construct a microbial interaction network (MIN). The MIN accounts for the associations formed and interactions among microbes and hosts. This work explores graph embedding feature selection through the construction of a sparse MIN using MAGMA embedded into a deep feedforward neural network (DFNN). This aims to reduce dimensionality and select prominent features that form the disease biomarkers. The selected features are passed through a deep forest classifier for disease prediction. The proposed methodology is experimentally cross-validated (5-fold) with different classifiers, existing works, and different models of MIN embedded in DFNN for the IBD and CRC datasets. Also, the selected biomarkers are verified against biological studies for the IBD and CRC datasets. The highest achieved AUC, accuracy, and f1-score are 0.863, 0.839, and 0.897, respectively, for the IBD dataset and 0.837, 0.768, and 0.757, respectively, for the CRC dataset. As observed, the proposed method is successful in selecting a subset of informative and prominent biomarkers for IBD and CRC.