In vitro tolerance induction of neonatal murine B cells as a probe for the study of B-cell diversification*

20Citations
Citations of this article
5Readers
Mendeley users who have this article in their library.
Get full text

Abstract

The susceptibility to in vitro tolerance induction has been implicated as a characteristic of B cells early in their development, since DNP-reactive B cells are tolerizable only during the first days after birth, and 25% of adult bone marrow cells are tolerizable. In the present study, a modification of the in vitro splenic focus technique was utilized to determine if PC-specific B cells, by virtue of their late expression (approximatelj 1 wk post-parturition), also display susceptibility to tolerance induction. The results demonstrate that at 7-10 days after birth, when over 90% of the DNP-specific splenic B cells are resistant to tolerance induction, the majority of PC-specific B cells are tolerizable. These results re-emphasize tolerance susceptibility as a characteristic of developing clones, confirm the late acquisition of PC-specific B cells, and support the contention that the acquisition of the specificity repertoire is a highly ordered, specifically predetermined process which is independent of antigen-driven events. © American Society for Clinical Pathology.

Cite

CITATION STYLE

APA

Metcalf, E. S., Sigal, N. H., & Klinman, N. R. (1977). In vitro tolerance induction of neonatal murine B cells as a probe for the study of B-cell diversification*. Journal of Experimental Medicine, 145(5), 1382–1386. https://doi.org/10.1084/jem.145.5.1382

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free