A CPG component LE generates depolarization of buccal neurons by producing constant plateau potentials during feeding responses of Aplysia kurodai

Abstract

In the buccal ganglia of Aplysia kurodai we have identified neurons (here termed LE neurons, or LE) producing plateau potentials lasting several seconds by application of short depolarizing currents. Results obtained from experiments using various bath solutions suggest that generation of these plateau potentials may be an endogenous property of LE. Application of various intensities or lengths of depolarizing currents induced in LE almost constant plateau potentials with fixed duration and depolarizing size. LE spikes produced monosynaptic EPSPs in the ipsilateral multi-action neuron (MA) and the jaw-closing motor neuron (JC) in the buccal ganglia. Conversely, MA spikes produced monosynaptic IPSPs in LE. There was electrical coupling between LE and both MA and JC. During the feeding-like response elicited by electrical stimulation of the nerve, LE showed rhythmic depolarization almost simultaneously with MA and JC, and firing on the plateau potentials occurred during the period of JC firing, the later phase of radula retraction. Hyperpolarization of LE during the feeding-like response suppressed generation of plateau potentials, though rhythmic small depolarization was still induced. During LE hyperpolarization, the duration of the depolarization of MA and JC was shortened. These results suggest that LE may be an element of the feeding CPG circuit and may contribute to part of the depolarization of MA and JC by generating constant plateau potentials during the feeding response, though LE may not have rhythm-generating ability. © 2006 Zoological Society of Japan.