Cryopreservation is an essential technique to preserve stem cells, semipermanently sustaining their potentials. There are two main approaches of cryopreservation for human pluripotent stem cells (hPSCs). The first is the vitrification, which involves instantaneous freeze and thaw of hPSCs. The second is the conventional slow-cooling method and a rapid thaw. Both cryopreservation protocols have been standardized and optimized to yield high survivability of hPSCs.
CITATION STYLE
Miyazaki, T., & Suemori, H. (2014). Cryopreservation of human pluripotent stem cells: A general protocol. Methods in Molecular Biology, 1235, 97–104. https://doi.org/10.1007/978-1-4939-1785-3_9
Mendeley helps you to discover research relevant for your work.