Determination of antibody structures

Abstract

While antibodies share a conserved structural framework, their complementarity-determining region loops are highly variable in size and sequence. Even more variable are the potential ways these loops can be used to interact with antigen. Thus, X-ray crystal structures of antibody Fab fragments and Fab-antigen complexes are critical for a detailed understanding of the antibody-antigen recognition process. This chapter describes the basic procedures necessary for the crystallization and structure determination of antibody Fab fragments by X-ray crystallography. © 2014 Springer Science+Business Media, New York.