This chapter is derived from our experience in the study of stress-Inducible Protein 1 (STI1) in extracellular vesicles. We used different techniques to isolate, explore, and characterize the extracellular vesicles that contained this protein. Ultracentrifugation and gel chromatography were used to isolate extracellular vesicles of different sizes, nanotracking particle analysis (NTA) determined number and size of vesicles, while flow cytometry and ELISA were used to determine the specific protein content of vesicles.
CITATION STYLE
Dias, M. V. S., Martins, V. R., & Hajj, G. N. M. (2016). Stress-Inducible Protein 1 (STI1): Extracellular vesicle analysis and quantification. In Methods in Molecular Biology (Vol. 1459, pp. 161–174). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3804-9_11
Mendeley helps you to discover research relevant for your work.