Fast screening procedure for antibiotics in wastewaters by direct HPLC-DAD analysis

Abstract

Growing concern about the contamination of wastewaters by antibiotics demands fast but sensitive analytical methodologies, for the screening of a large number of samples. The purpose of this work was to develop a simple methodology, using direct injection of the samples, by HPLC with diode array detection (DAD), for a multiresidue analysis of five antibiotics of different classes. Wastewater from an urban water treatment plant was selected as a model to study possible coelution of interfering compounds. The linearity interval ranged from 40 to 400 μg/ L for amoxicillin (Amox), metronidazole (Metro), cefazolin (Cefa), and chloramphenicol (Chloram) and from 20 to 200 μg/L for sulfamethoxazole (Sulfa), with LODs lower than 14 μg/L. Repeatability, expressed by the CV of six repeated injections, ranged from 1 to 8%, while the intermediate precision varied between 2 and 11%. The recovery ranged from 90 to 109%. This method enables the fast screening of a large number of samples, with an expanded uncertainty in the 1-22% range. The advantage of the proposed method is to significantly reduce the number of samples to be analyzed by more complex methods. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.