Phytochemical Evaluation, Antibacterial Activity and Bioactive Determination

Abstract

Objective: To find the antibacterial efficacy of the partially purified crude methanolic ext. of Cassia alata and to characterize the active compds. responsible for the activity. Method: Using methanol as solvent, the crude ext. was further subjected to qual., quant. and spectral studies using GC-MS, FT- IR and UV-VIS. Findings: Active principle compds. and antibacterial efficacy of the flower exts. of Cassia alata have been examd. against five bacterial strains of both pos. and neg. The partially purified crude methanolic exts. show a significant response against Staphylococcus aureus, Salmonella typhi but inactive against other strains such as P. aeruginosa, E. coli, P. vulgaris. The partially purified crude flower exts. of Cassia alata, shows a remarkable min. inhibitory concn. of 40mg/mL against S. typhi and S. aureus. However, the plant ext. obsd. to be bacteriostatic at a very low concn. with a min. bactericidal concn. against the Gram pos. organisms tested. The partially purified exts. show slightly above the min. inhibitory concn. nearly potent as std. antibiotic rifampicin and the inhibition ranges from 10 to 25 mm. The spectral unveilingphy to constituents such as fatty acid Me esters, flavonoids, steroids, terpenoids, phenolics, aldehydes, arom. compds. and alcs. can be postulated for its antibacterial efficacy of the partially purified exts. taken for this study. [on SciFinder(R)]