Determination of Coenzyme A (CoASH) in the presence of different thiols by using flow-injection with a UV/Vis spectrophotometric detector and potentiometrie determination of CoASH Using an Iodide ISE

Abstract

Coenzyme A (CoA or CoASH) is one of the most important biologically active compounds, and for this reason a reliable, fast and simple determination of this species is needed. We describe a simple and fast assay of CoASH using Potentiometrie flowinjection analysis and spectrophotometric kinetic determination. The described methods are suitable for use over a wide CoASH concentration range (1 x 10-6 - 1 x 10-4 M).