The tissue engineering method of decellularization and recellularization has been successfully used in a variety of regenerative medicine applications. The protocols used to de/recellularize various organs and tissues are largely different. Here we describe a method to effectively engineer a bioartificial colon by completely removing original cells from human intestinal tissues followed by repopulating the acellular tissue matrix with cell cultures. This method provides a novel approach for human intestinal regeneration and can be used to identify potential cancer driver genes.
CITATION STYLE
Chen, H. J., & Shuler, M. L. (2019). Engineering a Bioartificial Human Colon Model Through Decellularization and Recellularization. In Methods in Molecular Biology (Vol. 1907, pp. 91–102). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8967-6_7
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