Transcriptional regulation of gene expression constitutes a fundamental mechanism of many developmental processes. Therefore, identification and characterization of binding sites of transcription factors are important for uncovering the mechanisms of a particular developmental process. Here, we describe detailed procedures for genome-wide analysis of binding sites of a transcription factor involved in the fiber-type differentiation of skeletal muscle. By conducting ChIP-seq followed by a series of downstream analyses, in-depth information on binding sites of transcription factors can be obtained in a genome-wide manner. © 2013 Springer Science+Business Media New York.
CITATION STYLE
An, C. I., & Hagiwara, N. (2013). Genome-wide analysis of transcription factor-binding sites in skeletal muscle cells using ChIP-seq. Methods in Molecular Biology, 1067, 51–64. https://doi.org/10.1007/978-1-62703-607-8_4
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