Expression and regulation of neuromedin b in pituitary corticotrophs of male melanocortin 2 receptor-deficient mice

8Citations
Citations of this article
15Readers
Mendeley users who have this article in their library.

Abstract

The hypothalamic-pituitary-adrenal (HPA) axis is a major part of the neuroendocrine system that controls responses to stress, and has an important function in the regulation of various body processes. We previously created a mouse line deficient in the melanocortin 2 receptor (MC2R). MC2R-deficient mice (MC2R-/- mice) have high adrenocorticotropic hormone (ACTH) levels because of undetectable corticosterone levels. Increased neuromedin B (NMB) expression was recently reported in the pituitary gland of adrenalectomized mice, a model for acute adrenal insufficiency. To investigate gene expression in the pituitary gland under chronic adrenal deficiency, we examined the pituitary gland of MC2R-/- mice, a model of chronic adrenal insufficiency. To understand the molecular background of pituitary cells under chronic adrenal deficiency, we first performed DNA microarray analyses using the pituitary glands of the MC2R-/- mice. The DNA microarray analysis and real-time polymerase chain reaction showed that NMB expression was higher in the MC2R-/- than in the wild-type (WT) mice. We detected NMB expression in the MC2R-/- pituitary corticotrophs by immunohistochemistry using the specific antibodies for ACTH and NMB. In addition, the plasma NMB concentration was significantly higher in the MC2R-/- mice than in the WT mice. Subcutaneous implantation of a sustained-release corticosterone pellet decreased the expression of NMB mRNA as well as pituitary proopiomelanocortin mRNA. In isolated anterior pituitary cells, NMB mRNA expression was increased by the administration of corticotropin-releasing hormone (CRH) and was suppressed by dexamethasone treatment. In this study, we first demonstrate NMB expression in corticotrophs and its regulation by CRH and glucocorticoids. Furthermore, corticotrophs seemed to secrete NMB into the systemic circulation. Copyright © 2014 by the Endocrine Society.

References Powered by Scopus

Analysis of relative gene expression data using real-time quantitative PCR and the 2<sup>-ΔΔC</sup>T method

149980Citations
N/AReaders
Get full text

Structure and function of the blood-brain barrier

3951Citations
N/AReaders
Get full text

cDNA cloning, characterization, and brain region-specific expression of a neuromedin-B-preferring bombesin receptor

274Citations
N/AReaders
Get full text

Cited by Powered by Scopus

ACTH receptor (MC2R) specificity: What do we know about underlying molecular mechanisms?

64Citations
N/AReaders
Get full text

Pituitary transcriptomic study reveals the differential regulation of lncRNAs and mRNAs related to prolificacy in different FecB genotyping sheep

51Citations
N/AReaders
Get full text

Neuromedin B receptor disruption impairs adipogenesis in mice and 3T3-L1 cells

5Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Kameda, H., Miyoshi, H., Shimizu, C., Nagai, S., Nakamura, A., Kondo, T., … Atsumi, T. (2014). Expression and regulation of neuromedin b in pituitary corticotrophs of male melanocortin 2 receptor-deficient mice. Endocrinology, 155(7), 2492–2499. https://doi.org/10.1210/en.2013-2077

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 8

80%

Researcher 2

20%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 4

44%

Engineering 2

22%

Biochemistry, Genetics and Molecular Bi... 2

22%

Psychology 1

11%

Save time finding and organizing research with Mendeley

Sign up for free