Abstract
The targeted destruction of key regulators helps to drive the cell cycle. Here we describe a quantitative assay to measure destruction of different regulators in mitotic cells. This assay uses GFP-tagged substrates and time-lapse fluorescence microscopy of single cells to pinpoint the timing of destruction of different substrates at different stages in mitosis. © 2009 Humana Press, a part of Springer Science+Business Media, LLC.
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APA
Lindon, C., & Fiore, B. D. (2009). Measuring proteolysis in mitosis. Methods in Molecular Biology, 545, 259–270. https://doi.org/10.1007/978-1-60327-993-2_16
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