Detection and confirmation of hispolon in the mushroom Phellinus linteus

Abstract

Phellinus linteus (PL), a mushroom known as krathin phiman in Thai, has been used as a traditional medicine in the treatment of various cancers. Hispolon obtained from PL ethanol extracts is a promising anti-cancer compound. In this study, ethanol concentration and extraction time were varied for a soxhlet extraction. The content of hispolon in a crude extract was determined by high performance liquid chromatography with a UV detector. The presence of hispolon in the crude extract was demonstrated by liquid chromatography-tandem mass spectrometry (LC-MS/MS) with daughter ion scanned mode. The results showed that both hispolon and Thai PL have a similar parent ion at m=z 218.91 and daughter ions at m=z 176, 160, 135.