HPLC determination and pharmacokinetic study of homoeriodictyol-7-O-β- D-glucopyranoside in rat plasma and tissues

Abstract

Homoeriodictyol-7-O-β-D-glucopyranoside (HEDT-Glu) was isolated from Viscum coloratum and identified by MS, 1H- and 13C-NMR. A HPLC method was developed for determination of HEDT-Glu in rat plasma and tissues. All biological samples were pretreated by protein precipitation with acetone. Vanillin was selected as internal standard. The mobile phase consisted of methanol-water-glacial acetic acid (45 : 55 : 0.5, v/v/v). Good linearity were observed over the concentration ranges of 0.1-200.0 μg·ml -1 in rat plasma and 0.05-5.0 μg·ml-1 in tissues. Both intra- and inter-day precisions of HEDT-Glu, expressed as the relative standard deviation, were less than 13.1%. Accuracy, expressed as the relative error, ranged from -0.8 to 5.4% in plasma and from -5.6 to 9.4% in tissues. The mean extraction recovery of HEDT-Glu was above 73.17% in biological samples. The described assay method was successfully applied to the pre-clinical pharmacokinetic study of HEDT-Glu. After intravenous administration of HEDT-Glu to rat, AUC and CLtot were 16.04±3.19 μg·h·ml-1 and 0.85±0.17 l·kg -1·h-1, respectively. T1/2,α and t1/2,β were 0.06±0.01 h and 1.27±0.31 h, respectively. HEDT-Glu was cleared from the blood and mainly distributed to the liver and small intestine. © 2007 Pharmaceutical Society of Japan.