Histone modifications play an essential role in chromatin-associated processes including gene regulation and epigenetic inheritance. It is therefore very important to quantitatively analyze histone modifications at both the single gene and whole genome level. Here, we describe a robust chromatin immunoprecipitation (ChIP) method for Arabidopsis, which could be adapted for other plant species. This method is compatible with multiple downstream applications including qPCR, tilling arrays, and high-throughput sequencing. © Springer Science+Business Media New York 2014.
CITATION STYLE
Song, J., Rutjens, B., & Dean, C. (2014). Detecting histone modifications in plants. Methods in Molecular Biology, 1112, 165–175. https://doi.org/10.1007/978-1-62703-773-0_11
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