Modular organization of the silkmoth antennal lobe macroglomerular complex revealed by voltage-sensitive dye imaging

Abstract

We succeeded in clarifying the functional synaptic organization of the macroglomerular complex (MGC) of the male silkmoth Bombyx mori by optical recording with a voltage-sensitive dye. Sensory neurons in the antennae send their axons down either the medial nerve (MN) or lateral nerve (LN), depending on whether they are located on the medial or lateral flagella. Pheromone-sensitive fibers in the MN are biased towards the medial MGC, and those in the LN are biased towards the lateral MGC in the antennal lobe. In our optical recording experiments, the postsynaptic activities in the MGC were characterized by pharmacological analysis. Postsynaptic activities in the MGC were separated from sensory activities under Ca2+-free conditions, and subsequently the inhibitory postsynaptic activities were separated by applying bicuculline. We found that the inhibitory postsynaptic responses always preceded the postsynaptic responses separated under Ca2+-free conditions. Moreover, the excitatory postsynaptic activities were calculated by subtracting the inhibitory potentials from the posysynaptic activities separated under Ca2+-free conditions. When the MN was stimulated, the amplitudes of the excitatory postsynaptic activities in the central toroid, the medial toroid and the medial cumulus were selectively higher than those in the other areas. By contrast, when the LN was stimulated, excitatory postsynaptic activities were evoked in areas in both the lateral toroid and the lateral cumulus. The inhibitory postsynaptic activities were equally distributed throughout the whole MGC. These data suggest that there is a modular organization to the MGC such that information from the two main branches of the antenna is segregated to different sub-regions of the MGC glomeruli.