Rapid Detection of Hormonal Involvement in Light Responses

Abstract

Many aspects of light-controlled metabolism and development of plants depend on hormonal pathways. Here, a method is described to identify such hormonal dependence in light-regulated processes. A number of compounds—hormones and chemicals which interfere with hormonal pathways—are listed because of their usefulness in pharmacological treatment experiments. As an example for practical use of such compounds, elongation growth is discussed. An experimental setup is described in which plants are grown so that their structures develop predominantly in a two-dimensional plane. Time-lapse imaging is used to follow the plants in time, and image analysis reveals changes in plant morphology.