In Vitro Germination and Plantlet Establishment of Wild Chamomile of Morocco Cladanthus mixtus (L.) Oberpr. and Vogt

Abstract

In vitro seeds germination and\rplantlets establishment of Cladanthus mixtus (L.) Oberpr. and Vogt were\rstudied in this report. A reliable protocol was developed for in vitro seed germination, multiplication and plantlet regeneration of Cladanthus\rmixtus. The seeds were sterilized and cultured in different media. Among\rthe two basal media evaluated for symbiotic seed germination, gelled distilled\rwater was found to be the best with a high percentage of seeds germination\r(100%) after 33 hours of culture. Seedlings were further transferred to\rdifferent types of media. Result observed after 4 weeks showed that MS medium\rpromotes the highest growth with an average of 2.75 ± 0.12 cm shoot length and\r2.60 ± 0.29 shoots per explants, and the mean number of roots achieved 3.33 ± 0.17\rroot per explants with a length of 2.42 ± 0.16 cm. This study showed that macroelements\rof MS (1962) medium is essential for in vitro shoot multiplication,\rgrowth and rooting of shoots of Cladanthus mixtus L.