Virus-induced gene silencing (VIGS) is a powerful tool for rapidly knocking down the expression of plant genes to elucidate functional genomics. We have established a VIGS vector for monocot plants derived from Foxtail mosaic virus (FoMV), a positive-sense single-stranded RNA virus. For silencing a targeted gene, plant gene fragment was inserted into the vector between open reading frame 4 (ORF4) and ORF5 under the control of a duplicated coat protein promoter. Plants of different monocot species were infected by mechanical inoculation with sap from FoMV derivative-infected Chenopodium quinoa leaves. Gene silencing was typically observed within 2–3 weeks after inoculation. In this chapter, we describe the detailed protocol for silencing a target gene in various Poaceae plants by using FoMV-based vectors.
CITATION STYLE
Huang, Y. W., Chang, C. Y., & Hsu, Y. H. (2020). Virus-induced gene silencing in poaceae using a foxtail mosaic virus vector. In Methods in Molecular Biology (Vol. 2172, pp. 15–25). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0751-0_2
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