Massive Mutagenesis® is a proprietary library creation method that enables the fast generation of high-quality genetic libraries. Starting from a single gene on a plasmid and hundreds to thousands of oligonucleotides, a one-step single-strand circular amplification method creates random combinations of several site-directed substitutions, insertions, or deletions. Libraries of up to a billion such variants have been routinely generated. Sequencing those variants demonstrated lower biases than alternative approaches such as error-prone PCR. Screening and selecting them has yielded improved biocatalysts, therapeutic proteins, and antibodies. © 2010. Springer Science+Business Media, LLC.
CITATION STYLE
Sylvestre, J. (2010). Massive Mutagenesis®: High-throughput combinatorial site-directed Mutagenesis. Methods in Molecular Biology, 634, 233–238. https://doi.org/10.1007/978-1-60761-652-8_17
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