ATP-driven translocation of helicases along DNA can be assayed in several ways. Reagentless biosensors, based on fluorophore-protein adducts, provide convenient ways for real-time assays of both the separation of dsDNA and the hydrolysis of ATP. Single-stranded DNA can be assayed using a modified single-stranded DNA-binding protein (SSB), and phosphate production during ATP hydrolysis can be measured by a modified phosphate-binding protein. Advantages and limitations of these approaches are compared with those of other types of measurements. © 2009 Humana Press, a part of Springer Science+Business Media, LLC.
CITATION STYLE
Webb, M. R. (2010). Fluorescent biosensors to investigate helicase activity. Methods in Molecular Biology, 587, 13–27. https://doi.org/10.1007/978-1-60327-355-8_2
Mendeley helps you to discover research relevant for your work.