Heat was used to break up the mucilage-bound colonies of the blue-green alga, Microcystis aeruginosa Kiitz. emend. Elenkin, for cell counting. Samples were heated in a water bath at 80°C for 5-10 min, then agitated in a Vortex mixer for 30-60 s. The treatment separated cells completely and reaggregation of cells did not occur. There was no evidence that heating disintegrated the cells. © 1979 Taylor & Francis Group, LLC.
CITATION STYLE
Humphries, S. E., & Widjaja, F. (1979). A simple method for separating cells of microcystis aeruginosa for counting. British Phycological Journal, 14(4), 313–316. https://doi.org/10.1080/00071617900650331
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