Analysis of developmental control genes using virus-induced gene silencing

Abstract

A consistent challenge in studying the evolution of developmental processes has been the problem of explicitly assessing the function of developmental control genes in diverse species. In recent years, virusinduced gene silencing (VIGS) has proved to be remarkably adaptable and efficient in silencing developmental control genes in species across the angiosperms. Here we describe proven protocols for Nicotiana benthamiana and Papaver somniferum , representing a core and basal eudicot species. © Springer Science+Business Media New York 2013.