Determination of Bioactive Anti-Nonalcoholic Fatty Liver Effect Extracts From Phyllanthus Niruri Using Tri-Step Infrared Spectroscopy

Abstract

Phyllanthus niruri, belongs to Family of Phyllanthaceae, is a phytomedicine for liver related diseases such as hepatitis B, and jaundice. The aim of this study is to distinguish the fingerprint of P. niruri extract which active in ameliorating non-alcoholic fatty liver disease by tri-steps infrared spectroscopy, namely fourier transform infrared spectroscopy (FTIR), second derivative infrared spectroscopy (SD-IR), and two-dimensional correlation infrared spectroscopy (2D-IR). The structural information of the samples indicated that the P. niruri raw herb contains large amount of starch and protein, while the main components of methanolic and water extracts of P. niruri are esters and hydroxyl acids, respectively. However, the active 50% methanolic extract of P. niruri may has higher content of flavonoids and phenolic compounds. As a result, tri-steps infrared spectroscopic analysis not only can provide useful information regarding to the main chemical constituents of P. niruri, and its various extracts but also can be used as an analytical tool to distinguish activity of P. niruri extracts in anti-nonalcoholic fatty liver effect.