The chimeric antigen receptor (CAR) has been extensively exploited in cancer immunotherapy. In spite of the success of CAR T cells in clinical applications, the molecular mechanism underlying CAR-T cell activation remains unclear. Key questions remain: how are CARs activated by tumor antigens? How do activated CARs transduce signaling to downstream pathways? Here we introduce a microscopy-based method for studying CAR signaling. We use an antigen-coated supported lipid bilayer to activate CARs and combine it with TIRF microscopy to visualize the initial activation process of CAR T cells. This enables monitoring CAR signaling at high spatial and temporal resolutions.
CITATION STYLE
Libby, K. A., & Su, X. (2020). Imaging Chimeric Antigen Receptor (CAR) Activation. In Methods in Molecular Biology (Vol. 2111, pp. 153–160). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0266-9_13
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