The discovery of new enzymes is strongly enabled by the implementation of high-throughput screening methods to detect enzymatic activity in single organisms or clone expression libraries, or to benchmark their performances against known prototypes. In this chapter, a number of methods, applicable at high-throughput scale, are described that allow the screening and characterization of enzymes relevant to biotechnology, particularly, ester-hydrolases (esterases, lipases, phospholipases, and polyester hydrolases).
CITATION STYLE
Molina-Espeja, P., Fernandez-Lopez, L., Golyshin, P. N., & Ferrer, M. (2023). Assigning Functions of Unknown Enzymes by High-Throughput Enzyme Characterization. In Methods in Molecular Biology (Vol. 2555, pp. 181–194). Humana Press Inc. https://doi.org/10.1007/978-1-0716-2795-2_13
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