50 Hz Frequency Magnetic Field Effects On Pseudomonas Aeruginosa And Bacillus Subtilis Bacteria

Abstract

The effect of electromagnetic field of different intensities on Pseudomonas aeruginosa (as gram-negative bacteria) and Bacillus subtilis (as gram-positive bacteria) was investigated to find out the effective magnetic field strength that alters the running physiological processes of every microorganism. Equal volumes of P. aeruginosa and B. subtilis suspensions were exposed for one hour at their maximum rate of active growth to the electromagnetic field (2 -10 mT, 50 Hz). The results indicated that no remarkable differences were found in the growth of exposed P. aeruginosa. Moreover, a remarkable inhibition in the growth of exposed relative to unexposed B. subtilis cells was achieved at (4 mT) as compared with other intensities which may indicate that this magnetic field induction had a great effect on the biological activity of the cells, so more investigations were made at this magnetic field induction. Remarkable changes in the growth characteristics could be easily detected as the absorbance decreased which indicate a decrease in the cells number and consequently an inhibition case for the bacteria. Also, the antibiotic sensitivity test of B. subtilis cells indicated either inhibition or stimulation case for the bacteria depending on the drug mode of action.